![SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM. SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM.](https://cdn.numerade.com/ask_images/98e7f0d592204f928d39e0d8c9fe3086.jpg)
SOLVED: I need to calculate the primer concentration after PCR cycle. My DNA segment (798 bp) is amplified by PCR. The 50 uL mixture of PCR reaction. Primer concentration is 2 uM.
![Optimizations of primers and EvaGreen concentrations. To provide an... | Download Scientific Diagram Optimizations of primers and EvaGreen concentrations. To provide an... | Download Scientific Diagram](https://www.researchgate.net/publication/260117464/figure/fig2/AS:309801591558145@1450873863675/Optimizations-of-primers-and-EvaGreen-concentrations-To-provide-an-initial-optimization.png)
Optimizations of primers and EvaGreen concentrations. To provide an... | Download Scientific Diagram
![M Gradient PCR using Taq polymerase with 125ng primer concentration M: 1 kb ladder 1: 60°C 2: 62°C 3: 64.4°C 4:66.8°C 5: 69.2°C 6:71.5°C 284D. - ppt download M Gradient PCR using Taq polymerase with 125ng primer concentration M: 1 kb ladder 1: 60°C 2: 62°C 3: 64.4°C 4:66.8°C 5: 69.2°C 6:71.5°C 284D. - ppt download](https://slideplayer.com/10862899/39/images/slide_1.jpg)
M Gradient PCR using Taq polymerase with 125ng primer concentration M: 1 kb ladder 1: 60°C 2: 62°C 3: 64.4°C 4:66.8°C 5: 69.2°C 6:71.5°C 284D. - ppt download
![Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41467-022-29500-4/MediaObjects/41467_2022_29500_Fig1_HTML.png)
Designing highly multiplex PCR primer sets with Simulated Annealing Design using Dimer Likelihood Estimation (SADDLE) | Nature Communications
![Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers | BMC Biotechnology | Full Text Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers | BMC Biotechnology | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2F1472-6750-11-70/MediaObjects/12896_2011_Article_626_Fig2_HTML.jpg)
Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers | BMC Biotechnology | Full Text
![Diagnostics | Free Full-Text | Thermostable Heptaplex PCR Assay for the Detection of Six Respiratory Bacterial Pathogens Diagnostics | Free Full-Text | Thermostable Heptaplex PCR Assay for the Detection of Six Respiratory Bacterial Pathogens](https://www.mdpi.com/diagnostics/diagnostics-11-00753/article_deploy/html/images/diagnostics-11-00753-g001.png)
Diagnostics | Free Full-Text | Thermostable Heptaplex PCR Assay for the Detection of Six Respiratory Bacterial Pathogens
![Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays | BMC Research Notes | Full Text Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays | BMC Research Notes | Full Text](https://media.springernature.com/lw685/springer-static/image/art%3A10.1186%2F1756-0500-2-112/MediaObjects/13104_2008_Article_250_Fig2_HTML.jpg)
Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real-time PCR assays | BMC Research Notes | Full Text
![Unreacted Labeled PCR Primers Inhibit the Signal in a Nucleic Acid Lateral Flow Assay as Elucidated by a Transport Reaction Model | ACS Measurement Science Au Unreacted Labeled PCR Primers Inhibit the Signal in a Nucleic Acid Lateral Flow Assay as Elucidated by a Transport Reaction Model | ACS Measurement Science Au](https://pubs.acs.org/cms/10.1021/acsmeasuresciau.2c00005/asset/images/large/tg2c00005_0006.jpeg)